Learn how to extract dna from fruits and vegetables. It exists in everything from humans and animals to microorganisms and fruit. Mashing the banana exposes a greater surface area from which to extract the dna. A peeled kiwi fruit a blender surgical spirit or vodka washingup liquid table salt a strainer a glass tumbler 1 chill the surgical spirit by leaving it in the freezer overnight. Extract dna from a strawberry unlocking lifes code. The salt enables the dna strands to come together, or aggregate. Deoxyribonucleic acid dna is stored in every one of your cells and contains your bodys genetic material. Dna extraction, can also be used to alter animals, from making them glowinthedark. Students will learn how chemical substances can break. All the genetic information for a living organism is contained in its dna. You can collect the dna using a toothpick, chopstick or paperclip. Banana dna extraction lesson plan primary learning outcomes. Banana dna extraction science experiments for kids.
If you continue browsing the site, you agree to the use of cookies on this website. Extracting a dna sample from an orange requires only a few simple household products and items that can be purchased at a grocery store. These spirals are the backbone of the dna, and are made up of sugars and phosphates. This video shows how to extract dna from strawberries using everyday items. Dna can be extracted from cells by a simple technique with household chemicals, enabling students to see.
You can use powerpoint slides 48 to help illustrate this. All living things, bananas and people included, pass on information from one generation to the next using the same basic material, dna. Dna extraction from fruit ut southwestern medical center. In this step the detergent breaks down the cell membranes so the dna can be released. Extracting dna from fruit when we study reproduction in grade 9, we sometimes forget that plants have dna and rna, just like we do. We are going to use fruits because they are soft and easy to pulverize. Dna, or deoxyribonucleic acid, is the basic programming in the cells of all living organisms. Some kits may require you to use a stabilizing rinse immediately after scrubbing your cheeks. These kits help extract dna from particular cell types or sample types. We will use common household products to break apart the cells in a banana and extract out the dna. How to extract strawberry dna with pictures wikihow. Dna is found in the cells of every living organism. Create a saline solution in a beaker by adding two lab scoops of salt to approximately 25 ml of distilled water. Extracting dna from strawberries you will need, for one extraction.
Put about 50 ml of chilled ethanol into the 100ml beaker and place the beaker in the ice bath. To understand the important principles behind this step, it is not essential to do this experiment at this stage or, in fact, at all. His experiment involved bananas, kiwis, and strawberries. See lab handout strawberry dna extraction questions. There are numerous ways to extract dna, but many involve visiting a lab to submit a sample of blood or other bodily fluids. Strawberries are chosen for this particular experiment because strawberry cell has eight copies of the genome, and it would be easier to extract the dna since it is. Before you begin make sure the alcohol isopropyl alcohol is in the freezer getting cold and check each box after you complete each step. Most bodily fluids contain dna, including saliva, blood, semen, urine, and feces. Students will learn the simple method of extracting dna and why each step is necessary. The main problem is that much of the material that precipitates from kiwi fruit is probably pectin rather than dna. Answer any questions from the list made at the beginning of class.
Dna isolation is one of the most basic and essential techniques in the study of dna. If using a brush or swab, youll rub the collection device against the insides of your cheeks for approximately 30 seconds to a minute. Experiment sheet for the extraction of dna teaching. Dna determines the colour of your eyes and the col our of your hair. Slowly add the methylated spirits to the glass containing the oniondetergent mixture. In this activity, you will extract dna from banana, strawberry and kiwi fruits. We made a solution water, soap, and saltto free the dna from other components. Dna or deoxyribonucleic acid is a long molecule that is found in all living things. However, they can be expensive to use routinely, so many labs have their own methods for dna extraction. We will show you how to mix a solution that will take apart the strawberry cells and release the dna from inside them. Extracting dna from fruit biochemistry kids projects,molecular biology projects, biochemistry projects topics ideas, chemistry science fair project experiments for kids and also organics biochemistry, bio chemistry science ideas for cbse,icse,gcse,middleschool, elementary school for grade or class 5th,6th,7th,8th,9th,10th,11th,12th and high school students. Banana dna extraction science experiments elementary, 20 science experiments, science fair projects,science fair ideas experiments, kids project experiment ideas, science experiment projects, kids experiments science, children simple,cool,fun and easy science fair experiments,topics for science experiments, and also for middle school, elementary school for.
This week derek is in the department of developmental biology at cambridge university with scientist lucy wheatley and student helper, lucy brown. Hypothesis handsoap will extract more dna than the dish soap. Introduction this life science based experiment will test strawberries in their various stages of ripeness, in order to see which stage will yield the most extractable dna. Make sure that you take a picture of the dna you extracted. Remove the stick from the cup to see your glob of extracted dna. It will be fascinating to show students that we can extract dna from ordinary fruits, such as bananas, and vegetables, such as peas. Possible answerwe crushed the bananas to help release the dna. In this extraction activity we have the students compare the papaya from 4.
Dna can be extracted from anything living so you could also try this experiment with strawberries or bananas. Using dna from strawberries will help you have a successful dna preparation so you can purify a lot of dna. Artificial life viewing activity teacher notes extracting dna. This simple experiment will show you how to extract dna from fruit like a banana or strawberry. Remove the skin of the bananas and kiwi, we just want the insides. Extract dna from fruits and vegetables donorschoose project. To understand how dna extraction is done, you will extract dna from strawberries today. This activity will demonstrate how dna can be isolated from a strawberry using common household materials. To isolate dna from kiwi fruit and to observe how it looks like. A teaspoons of the dna extraction liquid into the bag with the strawberries. Stir gently trying to avoid making too many bubbles in the mixture. Extracting dna from fruit and vegetables could you help out with a protocol to extract dna using kiwi fruit. Feb 11, 20 in this experiment i will show you how to extract dna from fruit. Extracting dna from strawberries slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising.
The liquid soap is added to help break down cell membranes to release the dna. This activity may be used from elementary science to high school biology courses. Review article enzymatic extraction and clarification of. All dna depot kits are designed for 6 groups working in groups of 2 or 3.
You should see some white string almost cotton like strands begin to appear in the glass. All living things, from humans and mice to plants and. Carefully pour icecold ethanol into the tube, until it is. Pour the dna extraction solution detergent and salt solution from step 2 over the fruit until the total volume of fruit and liquid is about twice the volume of the mashed fruit alone. Once these membranes are broken apart, the dna is released from the cell. Add methylated spirits up from the bottom of the glass. Dna is a molecule in the form of a double helix two spirals twisting around each other. Let the solution sit for a few minutes and you should see more dna come out of the solution. Then alcohol is added to cause the dna to precipitate out. Oct, 2012 this is a worksheet that gives the method for the extraction of dna from fruit. This is to break up some of the cells and provide a large surface area over which to extract the dna.
Because strawberry cells each have eight copies of the genome in every cell. Take a picture of each important step during the experiment. Extracting dna from apples by rashawn ross on prezi. Slowly pour very cold alcohol down the side of the small cup. Dna contains instructions that direct the activities of cells and, ultimately, the body. Since dna exists inside of the cell nucleus, proteins have to be denatured in order to locate the dna. Dna is a major topic in the introduction to biology lecture course bio 100, so being familiar with this molecule is very important. Apr 21, 2017 extract dna from anything living introduction. Slowly pour very cold alcohol down the side of the.
We have used onions in the past but i believe it may be easier using kiwi fruit. It can easily be seen with the naked eye when collected from thousands of cells. Collect the dna by dipping the stick into the liquid and turning it like youre collecting cotton candy or twirling spaghetti. Instructions include safety procedures, 6 experiments, questions and answers. If you are not able to carry out this experiment, read through the details below and try to work.
Student supplies see next page for more supplies strawberries sealing plastic bags dish soap salt meat tenderizer. The extraction of dna from cells and its purification are of primary importance to the field of biotechnology and forensics. Dna extraction process dna is an incredibly small molecule, but in large quantities, it can be seen. Believe it or not, a kiwis genetic material is very similar to your own.
Make sure you remove the fruit skins as they are mostly dead and dont contain dna. Jun 08, 2010 extracting dna from strawberries slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Deoxyribonucleic acid dna is considered the genetic blueprint of all living things. Give to students as close to the start of the activity as possible. Extracting dna from fruit biochemistry or molecular. My students need lab supply for extracting dna from fruits and vegetables i will be teaching 7th and 8th grade science. A blue print is a detailed drawing or map that directs the construction of a building. It is incredibly small, but we can see it by extracting dna and isolating chains of it. The filtration step pouring the mixture through the strainer allows for the collection of the dna and other cellular substances. Extracting human dna in the classroom buccal cheek cells can be harvested painlessly and in sufficient quantity to visualise dna extracted in a simple 4step protocol we will be carrying out an optimised dna extraction and discussing kitchen chemistry alternatives to the materials used dna extraction based on. Have students complete the student response worksheet.
Add 2 teaspoons of the dna extraction buffer to the bag, zip it up and. How you collect your dna will vary, depending on the type of kit youve purchased. Materials used apples detergent handsoap rubbing alcohol 7090% graduated. Nearly every cell in a persons body has the same dna. I use this sheet to teach pupils to follow instructions and become more independent. Dna extraction can be used to modify plants, by isolating dna from organisms with desirable traits, such as resistance to pesticides, and injecting them into the genome of the plant. This is a worksheet that gives the method for the extraction of dna from fruit. This activity can be easily completed during a 1 hour lesson. Dna extraction c 21 chemistry in the k8 classroom grades 48 2007, omsi orpapaya or pineapple juice.
Without swallowing, drink a mouthful of the solution from the paper cup and swish it back and forth for at least 30 seconds, occasionally. The dna will come out of solution at the boundary layer between the water and alcohol. When an organism has eight copies, called an octoploid, it has a lot more dna per cell than an organism that only has one copy. It is an experiment that can be performed both at home and in a school laboratory. Summarize the main steps involved in extracting dna from bananas. See and touch the genetic material that youll extract from the cells of a kiwi fruit. Use 1 cup of fresh, frozen diluted as directed, or canned juice.
How to extract dna at home kids science fair project. You could use other fruit or vegetable if you have students with allergies. Make sure juice contains raw, uncooked fruit juice. Half fill a small cup with the liquid from the jug. If you want to save your dna you can store it in a small container filled with the rubbing alcohol. Outreach activity extracting dna from kiwi fruit the node. Extracting dna from apples question if extracting the dna from household chemicals like handsoap and dish soap which would extract more.
Extracting dna from fruit biochemistry or molecular biology. Extracting dna from fruit in various stages of ripeness. Dna is a double stranded macromolecule composed of nucleotide bases pairing adenine with thymine and guanine with cytosine. All you need are some fruit and some things you probably have around the house right now. An extraction kit will be designed from common household items, such as salt and detergent, in order to purify the dna so that it is visible to the naked eye and can be weighed. Resea the bag and gently smash for another minute avoid making too many soap bubbles. Dna extraction buffer, calibrated transfer pipets, microtip transfer pipets, wooden sticks, large test tubes, small test tubes. Experiment sheet for the extraction of dna teaching resources. Pdf because dna degradation is mediated by secondary plant products such as. Prepare a hot water bath by putting about 5 to 8 cm 3 to 5 inches of hot water into a saucepan. Understand dna is in the cells of all living organisms including in the foods we eat. We use fruit for this exercise because they are often polyploid, meaning they.
Students will learn how chemical substances can break up the cell structures surrounding dna and why it is important for. Dna deoxyribonucleic acid is the hereditary material that directs the construction of living things. Clean up your mess by washing the test tubes and beaker. You can collect the dna using a toothpick, chopstick or. Pdf a simple and rapid method for isolation of high quality. If you took all of the dna out of some middlesized organism or part of an organism, like a piece of fruit, you could see and even touch dna. You dont want the skin because its mostly dead and doesnt have much dna in it.
Dna extraction from fruit adapted from isolation of dna from onionwoodrow wilson 1993 loretta loykasek, burleson high school, burleson, tx purpose to allow students to observe the dna found in plant cells. Because dna is in every cell, there is a lot of it in an organism. The gauze will catch cell debris and unmashed pieces of fruit. Activity overview helping you discover more about dna. Fruit dna extraction instructions partners in research. Dna extraction and gel electrophoresis introduction dna extraction and separation by agarose gel electrophoresis is a simple and exciting process that anyone can perform. A simple practical instruction worksheet to guide gcse biology students through extraction of dna from kiwi fruit. Dish detergent 112 cup water plastic cups coffee filter 12. During the dna extraction, the soap pulls apart the fats lipids and proteins that make up the membranes surrounding the cell and nucleus. Below is more information on dna, and how the experiment works to extract and make visible this tiny molecule. Notes and hints keep the isopropyl alcohol very colduse the freezer or ice bucket. This lab activity will allow you to extract dna from fruit in this case, clementines. Though it is much too small to see on its own, it is visible to the human eye en masse.
Within every living organism, most cells contain a complete set of dna instructions. Heres a simple experiment to extract and visualise the dna from kiwi fruit. Dna extraction and gel electrophoresis introduction. Extracting dna from strawberries linkedin slideshare. For dna extraction, detergent is used to lyse the cell so that dna is released into the solution. Biology of extracting dna from kiwi fruit teaching resources. Extracting dna from a in 10 easy steps before you begin make sure the alcohol isopropyl alcohol is in the freezer getting cold and check each box after you complete each step. Dna can be extracted from cells for scientific use.
Boyd extracting dna from fruit in stages of ripeness. In this article, i describe a simple experiment that will allow you to extract a bit of dna from a banana, however, you can also try it using other fruits and even vegetables. However, the high cost of specialized equipment and chemicals often hinder such an experiment from being carried by high school students. Dec 02, 2014 in this episode we are extracting the dna from a strawberry.
The kiwi needs to be broken up so the extra ction solution can get to the cells. Strawberry dna extraction institute for molecular bioscience. This gets rid of the fruit pulp and seeds and should leave a pure solution of dna. How to extract dna from a kiwi fruit experiments naked. Pour the filtered fruit liquid into the clean test tube. You can use a glass rod or wooden stick to spool the dna.
The soap solution contains sodium laurel sulfate, which can break up fats and proteins. Fruitful dna extraction 62 to do and notice extracting the dna prepare an ice water bath by putting ice and water into a saucepan or similar container to a depth of 5 to 8 cm. Since dna is the blueprint for life, everything living contains dna. The dna precipitates out of the solution when the alcohol is added. Why use strawberries to test your dna extraction kit. All the dna in a cell is known as a genome and provides the instructions to make that living thing function. Strawberry dna extraction all living things have dna. The experiment described below allows you to isolate dna from fruit. Now the group is familiar with dna, explain that they are going to extract dna from some fruit.
A kiwi fruit an onion will do if you dont have a kiwi 5g washing up liquid or hand soap 2g salt 100ml tap water 100ml of ice cold alcohol white rum or methylated spirits are best. During the dna extraction, the soap pulls apart the fats lipids and proteins that make up the membranes surrounding the. Learning how to extract and visualize dna from organisms is a basic procedure in molecular biology. Extracting dna from fruit instructions mix the washingup liquid, the water and the salt in one cup. If you want to save your fruit dna, you can store it in a small container filled with rubbing alcohol. This simple method allows you to extract dna from a strawberry and view it.
The dna will pass through the gauze into the test tube. To see dna with my own eyes and understand that it exists in all cells even though it is plant or food. In a separate bowl, mix the washing up liquid, salt and tap water. Dna extraction from fruit dna is the hereditary material in all cells. If you are not able to carry out this experiment, read. Students will observe first hand that dna is in the food they eat. To observe first hand that dna is in the food we eat to learn the simple method of dna extraction to explain the reason behind each step use of the microscope learning outcomes able to successfully extract dna from fruit able to see the extracted dna using a microscope materials part 1 kiwi fruit ziplock bag 250ml concial flask strainer funnel. In a separate cup, mash the fruit and a little water into a pulp. The actual laboratory activity allows students to determine if dna is in the food we eat by attempting to extract dna from foods such as strawberries.
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